Abstract
The present study was conducted to examine the antioxidant and antimicrobial activities of essential oil extracts from ischolar_mains of Viburnum nervosum (guch) collected from Sudhan Galli, District Muzaffarabad, Azad Jammu & Kashmir at an altitude of 7000 feet. The essential oil yields from this plant were found to be 0.025 % (w/w). GC and GC–MS analysis of the guch essential oil revealed the presence of 23 components, of which 15 were identified, representing 98% of the essential oil. The eudesmol (30.34%), caryophyllene oxide (17%), linalool (12.60%), spathulenol (10.70%), and ledene (9.80%) were the major components. The guch extracts contained appreciable level of total phenolic contents (628.26-968.04 GAE, mg/100g), total flavonoid contents (376.75-682.87 CE, mg/100g). Essential oil also exhibited good DPPH radical scavenging activity showing IC50 33.32, 25.65,23.07, 25.13, 22.97 µg/mL, and inhibition of peroxidation 19.23 and 93.04%, respectively. Of the Viburnum nervosum essential oil and extracts tested 80% ethanol exhibited maximum antioxidant activity. Essential oil showed appreciable mild antimicrobial activity against selected strains of bacteria and pathogenic fungi, as assessed by disc diffusion assay. The antioxidant activity of essential oil and extracts of demonstrated significant (p < 0.05) variations in results.
